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Series GSE63274 Query DataSets for GSE63274
Status Public on Sep 11, 2015
Title Intra-amniotic Balloon Inflation Induces an Inflammatory “Pulse” and Labor in Nonhuman Primates
Platform organism Macaca mulatta
Sample organism Macaca nemestrina
Experiment type Expression profiling by array
Summary Uterine stretch is thought to induce preterm labor in women with twin and higher order pregnancies, but the pathophysiology remains unclear. We investigated the pathogenesis of stretch-induced preterm birth for the first time in a pregnant nonhuman primate model.
Overall design Eleven chronically catheterized pregnant monkeys (Macaca nemestrina) at 118-125 days gestation (term=172 days) received either: 1) inflation of intra-amniotic balloons (N=6) or 2) saline inoculation (N=5). Cesarean section and fetal necropsy was performed due to preterm labor or to collect tissues, except in one case where the animal delivered spontaneously, reducing samples for microarray analysis to ten (five stretch and five control animals). Amniotic fluid and maternal plasma were analyzed for multiple cytokines and prostaglandins using Luminex, enzyme-linked immunosorbent assay and Analysis of Covariance. Ribonucleic acid was extracted from the myometrium in the lower uterus at Cesarean section and analyzed by microarray and quantitative reverse transcriptase polymerase chain reaction.
Contributor(s) Adams Waldorf KM, Mohan AR, Young RC, Ngo L, Das A, Tsai J, Bansal A, Paolella L, Soorana SR, Gough M, Astley C, Vogel K, Baldessari AE, Bammler TK, MacDonald JW, Gravett MG, Rajagopal L, Johnson M
Citation(s) 26284599
Submission date Nov 13, 2014
Last update date Dec 14, 2015
Contact name James William MacDonald
Organization name University of Washington
Department Environmental and Occupational Health Sciences
Street address 4225 Roosevelt Way NE
City Seattle
State/province WA
ZIP/Postal code 98105-6099
Country USA
Platforms (1)
GPL3535 [Rhesus] Affymetrix Rhesus Macaque Genome Array
Samples (10)
GSM1544977 Stretch_No_1
GSM1544978 Stretch_Yes_1
GSM1544979 Stretch_No_2
BioProject PRJNA267264

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Supplementary file Size Download File type/resource
GSE63274_RAW.tar 44.7 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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